Xenon in PDB 3pk4: Urate Oxidase Under 3.2 Mpa / 32 Bars Pressure of Xenon

All present enzymatic activity of Urate Oxidase Under 3.2 Mpa / 32 Bars Pressure of Xenon:
1.7.3.3;

The structure of Urate Oxidase Under 3.2 Mpa / 32 Bars Pressure of Xenon, PDB code: 3pk4 was solved by G.Marassio, N.Colloc'h, T.Prange, J.H.Abraini, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.76 / 1.85
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	80.405, 96.311, 105.593, 90.00, 90.00, 90.00
R / Rfree (%)	15.9 / 19.3

The structure of Urate Oxidase Under 3.2 Mpa / 32 Bars Pressure of Xenon also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Xenon atom in the Urate Oxidase Under 3.2 Mpa / 32 Bars Pressure of Xenon (pdb code 3pk4). This binding sites where shown within 5.0 Angstroms radius around Xenon atom.
In total 2 binding sites of Xenon where determined in the Urate Oxidase Under 3.2 Mpa / 32 Bars Pressure of Xenon, PDB code: 3pk4:
Jump to Xenon binding site number: 1; 2;

Xenon binding site 1 out of 2 in the Urate Oxidase Under 3.2 Mpa / 32 Bars Pressure of Xenon


Mono view


Stereo pair view

A full contact list of Xenon with other atoms in the Xe binding site number 1 of Urate Oxidase Under 3.2 Mpa / 32 Bars Pressure of Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Xe801 b:38.1 occ:1.00 CD1 A:LEU252 3.9 20.8 1.0 CG2 A:THR230 4.0 21.4 1.0 CD1 A:PHE219 4.1 21.3 1.0 CB A:THR230 4.1 21.5 1.0 CD2 A:LEU178 4.2 22.8 1.0 CG1 A:VAL227 4.3 18.9 1.0 CE1 A:PHE219 4.4 20.9 1.0 CE A:MET234 4.4 24.8 1.0 O A:VAL227 4.4 18.6 1.0 CA A:VAL227 4.5 18.5 1.0 CG2 A:THR180 4.7 20.0 1.0 CG2 A:VAL227 4.8 18.1 1.0 CB A:VAL227 4.8 16.8 1.0 N A:MET231 4.8 20.7 1.0 CG A:LEU178 4.9 20.4 1.0 OG1 A:THR230 5.0 20.4 1.0 C A:VAL227 5.0 18.2 1.0

Xenon binding site 2 out of 2 in the Urate Oxidase Under 3.2 Mpa / 32 Bars Pressure of Xenon


Mono view


Stereo pair view

A full contact list of Xenon with other atoms in the Xe binding site number 2 of Urate Oxidase Under 3.2 Mpa / 32 Bars Pressure of Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Xe802 b:34.8 occ:0.27 CE A:MET32 3.6 36.9 1.0 O A:TYR30 3.7 19.4 1.0 O A:VAL15 3.7 16.2 1.0 SD A:MET32 3.7 33.8 1.0 CG1 A:VAL15 3.8 20.7 1.0 N A:LYS17 3.8 16.8 1.0 CG A:MET32 3.9 25.4 1.0 CB A:LYS17 3.9 18.3 1.0 CG2 A:VAL73 4.0 21.7 1.0 CB A:TYR30 4.0 19.5 1.0 C A:VAL15 4.0 16.5 1.0 CB A:VAL15 4.1 17.5 1.0 C A:TYR30 4.1 18.1 1.0 CG1 A:VAL73 4.1 21.1 1.0 CB A:VAL73 4.1 21.4 1.0 C A:TYR16 4.1 16.0 1.0 CA A:TYR16 4.3 16.0 1.0 N A:TYR16 4.3 16.1 1.0 CB A:MET32 4.4 17.6 1.0 N A:MET32 4.4 15.3 1.0 CA A:GLU31 4.5 15.6 1.0 CA A:LYS17 4.5 18.4 1.0 N A:GLU31 4.5 17.1 1.0 C A:GLU31 4.7 15.8 1.0 CA A:TYR30 4.7 19.1 1.0 O A:TYR16 4.7 16.1 1.0 CA A:VAL15 4.8 16.2 1.0

G.Marassio, T.Prange, H.N.David, J.Sopkova-De Oliveira Santos, L.Gabison, N.Delcroix, J.H.Abraini, N.Colloc'h. Pressure-Response Analysis of Anesthetic Gases Xenon and Nitrous Oxide on Urate Oxidase: A Crystallographic Study. Faseb J. V. 25 2266 2011.
ISSN: ISSN 0892-6638
PubMed: 21421845
DOI: 10.1096/FJ.11-183046