Xenon in PDB 3pjk: Urate Oxidase Under 1.0 Mpa / 10 Bars Pressure of Xenon

All present enzymatic activity of Urate Oxidase Under 1.0 Mpa / 10 Bars Pressure of Xenon:
1.7.3.3;

The structure of Urate Oxidase Under 1.0 Mpa / 10 Bars Pressure of Xenon, PDB code: 3pjk was solved by G.Marassio, N.Colloc'h, T.Prange, J.H.Abraini, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.45 / 1.70
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	80.117, 96.183, 105.463, 90.00, 90.00, 90.00
R / Rfree (%)	16.7 / 19.2

The structure of Urate Oxidase Under 1.0 Mpa / 10 Bars Pressure of Xenon also contains other interesting chemical elements:

Chlorine	(Cl)	1 atom
Sodium	(Na)	1 atom

The binding sites of Xenon atom in the Urate Oxidase Under 1.0 Mpa / 10 Bars Pressure of Xenon (pdb code 3pjk). This binding sites where shown within 5.0 Angstroms radius around Xenon atom.
In total 2 binding sites of Xenon where determined in the Urate Oxidase Under 1.0 Mpa / 10 Bars Pressure of Xenon, PDB code: 3pjk:
Jump to Xenon binding site number: 1; 2;

Xenon binding site 1 out of 2 in the Urate Oxidase Under 1.0 Mpa / 10 Bars Pressure of Xenon


Mono view


Stereo pair view

A full contact list of Xenon with other atoms in the Xe binding site number 1 of Urate Oxidase Under 1.0 Mpa / 10 Bars Pressure of Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Xe801 b:35.0 occ:0.60 CD1 A:LEU252 3.8 17.1 1.0 CG2 A:THR230 3.9 15.9 1.0 CD1 A:PHE219 4.0 16.8 1.0 CB A:THR230 4.1 16.3 1.0 CD2 A:LEU178 4.2 17.0 1.0 CG1 A:VAL227 4.2 13.4 1.0 CE1 A:PHE219 4.2 15.6 1.0 CE A:MET234 4.4 20.1 1.0 O A:VAL227 4.5 13.1 1.0 CA A:VAL227 4.5 13.3 1.0 CG2 A:THR180 4.7 14.5 1.0 CB A:VAL227 4.8 12.4 1.0 N A:MET231 4.8 15.0 1.0 CG A:LEU178 4.8 15.3 1.0 CG2 A:VAL227 4.8 13.1 1.0 OG1 A:THR230 4.9 16.5 1.0 C A:VAL227 5.0 12.4 1.0

Xenon binding site 2 out of 2 in the Urate Oxidase Under 1.0 Mpa / 10 Bars Pressure of Xenon


Mono view


Stereo pair view

A full contact list of Xenon with other atoms in the Xe binding site number 2 of Urate Oxidase Under 1.0 Mpa / 10 Bars Pressure of Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Xe802 b:32.1 occ:0.10 CE A:MET32 2.1 17.1 0.4 CE A:MET32 3.3 25.4 0.6 SD A:MET32 3.4 18.7 0.4 CG A:MET32 3.6 13.7 0.4 O A:VAL15 3.6 11.6 1.0 SD A:MET32 3.6 22.2 0.6 O A:TYR30 3.7 14.7 1.0 CG1 A:VAL15 3.7 15.4 1.0 CG A:MET32 3.7 16.8 0.6 N A:LYS17 3.9 12.0 1.0 CG2 A:VAL73 4.0 17.2 1.0 C A:VAL15 4.0 11.9 1.0 CB A:TYR30 4.0 14.9 1.0 CB A:LYS17 4.0 12.5 1.0 CB A:VAL15 4.0 13.5 1.0 C A:TYR30 4.1 14.0 1.0 CG1 A:VAL73 4.1 16.9 1.0 CB A:VAL73 4.1 16.8 1.0 C A:TYR16 4.2 11.9 1.0 N A:MET32 4.3 10.8 1.0 CA A:TYR16 4.3 11.8 1.0 N A:TYR16 4.4 11.6 1.0 CB A:MET32 4.4 13.6 1.0 CA A:GLU31 4.4 11.5 1.0 N A:GLU31 4.5 12.8 1.0 C A:GLU31 4.6 11.5 1.0 CA A:LYS17 4.6 13.3 1.0 CA A:TYR30 4.7 14.4 1.0 CA A:VAL15 4.7 12.2 1.0 O A:TYR16 4.8 12.4 1.0 O A:HOH595 5.0 42.8 1.0 CA A:MET32 5.0 12.2 1.0

G.Marassio, T.Prange, H.N.David, J.Sopkova-De Oliveira Santos, L.Gabison, N.Delcroix, J.H.Abraini, N.Colloc'h. Pressure-Response Analysis of Anesthetic Gases Xenon and Nitrous Oxide on Urate Oxidase: A Crystallographic Study. Faseb J. V. 25 2266 2011.
ISSN: ISSN 0892-6638
PubMed: 21421845
DOI: 10.1096/FJ.11-183046