Xenon in PDB 1c6n: T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon

All present enzymatic activity of T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon:
3.2.1.17;

The structure of T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon, PDB code: 1c6n was solved by M.L.Quillin, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	60.00 / 2.20
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	61.166, 61.166, 97.068, 90.00, 90.00, 120.00
R / Rfree (%)	n/a / n/a

The structure of T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Xenon atom in the T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon (pdb code 1c6n). This binding sites where shown within 5.0 Angstroms radius around Xenon atom.
In total 4 binding sites of Xenon where determined in the T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon, PDB code: 1c6n:
Jump to Xenon binding site number: 1; 2; 3; 4;

Xenon binding site 1 out of 4 in the T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon


Mono view


Stereo pair view

A full contact list of Xenon with other atoms in the Xe binding site number 1 of T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Xe500 b:36.0 occ:0.92 CB A:ALA99 3.7 19.7 1.0 O A:LEU84 3.9 19.4 1.0 CB A:TYR88 4.0 10.4 1.0 CB A:VAL87 4.1 23.4 1.0 N A:TYR88 4.1 13.0 1.0 CD2 A:LEU118 4.1 27.9 1.0 CA A:TYR88 4.3 14.8 1.0 CG1 A:VAL87 4.3 10.4 1.0 CD2 A:LEU84 4.4 18.1 1.0 C A:VAL87 4.5 12.5 1.0 C A:LEU84 4.6 15.5 1.0 CA A:LEU84 4.7 21.7 1.0 CD2 A:TYR88 4.7 17.0 1.0 CB A:LEU84 4.7 20.5 1.0 CD1 A:LEU91 4.7 16.9 1.0 CA A:VAL87 4.8 18.0 1.0 CG A:TYR88 4.9 22.3 1.0 CG A:LEU84 5.0 27.4 1.0

Xenon binding site 2 out of 4 in the T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon


Mono view


Stereo pair view

A full contact list of Xenon with other atoms in the Xe binding site number 2 of T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Xe501 b:32.0 occ:0.62 CG1 A:VAL111 3.8 30.1 1.0 CD2 A:LEU118 3.9 27.9 1.0 CB A:PHE114 4.0 22.6 1.0 CD1 A:LEU121 4.0 22.2 1.0 CE A:MET102 4.1 29.5 1.0 CD2 A:LEU133 4.1 19.3 1.0 SD A:MET102 4.1 33.0 1.0 CG A:LEU118 4.3 24.1 1.0 CB A:SER117 4.5 11.6 1.0 XE A:XE502 4.5 35.6 0.6 O A:VAL111 4.5 16.5 1.0 CG A:PHE114 4.5 22.1 1.0 CD2 A:PHE114 4.7 29.7 1.0 N A:LEU118 4.7 12.4 1.0 O A:PHE114 4.9 31.5 1.0 CA A:LEU118 5.0 16.1 1.0

Xenon binding site 3 out of 4 in the T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon


Mono view


Stereo pair view

A full contact list of Xenon with other atoms in the Xe binding site number 3 of T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Xe502 b:35.6 occ:0.60 CD1 A:LEU121 3.7 22.2 1.0 CB A:ALA153 3.7 15.9 1.0 N A:ALA99 3.9 17.9 1.0 CA A:ALA99 3.9 23.8 1.0 CB A:ALA99 4.0 19.7 1.0 C A:ALA98 4.1 27.4 1.0 CB A:ALA98 4.1 14.8 1.0 SD A:MET102 4.2 33.0 1.0 O A:ALA98 4.2 20.4 1.0 O A:ARG95 4.4 29.1 1.0 XE A:XE501 4.5 32.0 0.6 CA A:ALA98 4.8 17.3 1.0 CG A:LEU121 4.9 25.6 1.0 CD2 A:LEU121 5.0 25.9 1.0

Xenon binding site 4 out of 4 in the T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon


Mono view


Stereo pair view

A full contact list of Xenon with other atoms in the Xe binding site number 4 of T4 Lysozyme Mutant C54T/C97A/L99A/F153A in the Presence of 8 Atm Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Xe503 b:20.0 occ:0.10 CB A:ALA153 3.5 15.9 1.0 N A:ARG154 3.5 19.3 1.0 C A:ALA153 3.5 27.6 1.0 CD1 A:LEU133 3.6 19.9 1.0 CA A:ARG154 3.7 15.8 1.0 O A:ALA153 3.7 19.9 1.0 CB A:ALA129 3.7 10.0 1.0 CB A:ALA130 3.7 25.4 1.0 O A:TRP126 3.7 16.5 1.0 N A:ALA130 3.7 22.1 1.0 O A:ILE150 3.8 21.5 1.0 CA A:ALA130 3.9 22.9 1.0 CB A:ARG154 3.9 19.4 1.0 CA A:ALA153 4.0 19.8 1.0 CD2 A:LEU121 4.1 25.9 1.0 C A:ALA129 4.1 15.9 1.0 CG A:ARG154 4.3 36.8 1.0 CG2 A:ILE150 4.4 8.4 1.0 O A:ALA129 4.6 16.7 1.0 CA A:ALA129 4.6 13.3 1.0 C A:TRP126 4.8 14.9 1.0 C A:ILE150 4.8 21.7 1.0 CG A:LEU133 4.8 20.0 1.0 N A:ALA153 4.9 13.2 1.0 CA A:ILE150 4.9 13.7 1.0

M.L.Quillin, W.A.Breyer, I.J.Griswold, B.W.Matthews. Size Versus Polarizability in Protein-Ligand Interactions: Binding of Noble Gases Within Engineered Cavities in Phage T4 Lysozyme. J.Mol.Biol. V. 302 955 2000.
ISSN: ISSN 0022-2836
PubMed: 10993735
DOI: 10.1006/JMBI.2000.4063