Xenon in PDB 1c68: T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon

All present enzymatic activity of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon:
3.2.1.17;

The structure of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon, PDB code: 1c68 was solved by M.L.Quillin, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	60.00 / 2.50
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	61.126, 61.126, 96.716, 90.00, 90.00, 120.00
R / Rfree (%)	n/a / n/a

The structure of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Xenon atom in the T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon (pdb code 1c68). This binding sites where shown within 5.0 Angstroms radius around Xenon atom.
In total 3 binding sites of Xenon where determined in the T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon, PDB code: 1c68:
Jump to Xenon binding site number: 1; 2; 3;

Xenon binding site 1 out of 3 in the T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon


Mono view


Stereo pair view

A full contact list of Xenon with other atoms in the Xe binding site number 1 of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Xe500 b:68.0 occ:0.67 XE A:XE501 3.0 58.0 0.4 XE A:XE502 3.0 59.9 0.4 CD2 A:PHE153 3.6 35.7 1.0 CB A:ALA133 3.7 4.3 1.0 CG A:PHE153 3.8 14.6 1.0 CE2 A:PHE153 3.9 45.6 1.0 O A:ALA129 4.1 25.7 1.0 CD1 A:PHE153 4.2 11.9 1.0 CZ A:PHE153 4.3 28.7 1.0 CB A:PHE153 4.3 17.6 1.0 CE1 A:PHE153 4.5 23.5 1.0 CB A:SER117 4.5 16.5 1.0 C A:ALA129 4.7 34.0 1.0 CG1 A:ILE150 4.7 9.8 1.0 CA A:ALA130 4.8 15.4 1.0 SD A:MET102 4.9 30.2 1.0 OG A:SER117 5.0 20.6 1.0

Xenon binding site 2 out of 3 in the T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon


Mono view


Stereo pair view

A full contact list of Xenon with other atoms in the Xe binding site number 2 of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Xe501 b:58.0 occ:0.41 CD1 A:LEU118 2.5 13.8 1.0 XE A:XE500 3.0 68.0 0.7 OG A:SER117 4.0 20.6 1.0 CZ A:PHE153 4.0 28.7 1.0 CG A:LEU118 4.0 38.9 1.0 CB A:SER117 4.0 16.5 1.0 SD A:MET102 4.1 30.2 1.0 CE2 A:PHE153 4.1 45.6 1.0 CB A:PHE114 4.1 22.6 1.0 N A:LEU118 4.2 34.6 1.0 CA A:LEU118 4.3 35.4 1.0 CE A:MET102 4.4 19.9 1.0 C A:SER117 4.5 36.3 1.0 CB A:LEU118 4.6 40.8 1.0 CG A:PHE114 4.7 21.2 1.0 CE1 A:PHE153 4.7 23.5 1.0 O A:PHE114 4.7 25.1 1.0 CD2 A:PHE114 4.8 18.6 1.0 CB A:ALA121 4.8 43.3 1.0 CD2 A:PHE153 4.8 35.7 1.0 O A:SER117 4.9 42.6 1.0 CB A:ALA133 5.0 4.3 1.0 CA A:SER117 5.0 21.1 1.0

Xenon binding site 3 out of 3 in the T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon


Mono view


Stereo pair view

A full contact list of Xenon with other atoms in the Xe binding site number 3 of T4 Lysozyme Mutant C54T/C97A/L121A/L133A in the Presence of 8 Atm Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Xe502 b:59.9 occ:0.37 XE A:XE500 3.0 68.0 0.7 CB A:PHE153 3.3 17.6 1.0 CG A:PHE153 3.6 14.6 1.0 N A:ARG154 3.7 20.9 1.0 CD1 A:PHE153 3.7 11.9 1.0 CB A:ALA130 3.7 10.9 1.0 CA A:ALA130 3.9 15.4 1.0 C A:PHE153 3.9 31.9 1.0 O A:TRP126 3.9 45.8 1.0 N A:ALA130 4.0 12.0 1.0 CA A:ARG154 4.0 24.7 1.0 O A:ILE150 4.0 30.2 1.0 CB A:ARG154 4.2 24.4 1.0 C A:ALA129 4.3 34.0 1.0 CA A:PHE153 4.3 10.3 1.0 O A:PHE153 4.4 33.0 1.0 CB A:ALA129 4.4 27.3 1.0 CD2 A:PHE153 4.4 35.7 1.0 CG2 A:ILE150 4.5 26.2 1.0 O A:ALA129 4.5 25.7 1.0 CE1 A:PHE153 4.7 23.5 1.0 CA A:ILE150 4.8 18.9 1.0 C A:ILE150 4.8 18.6 1.0 C A:TRP126 4.9 35.0 1.0 CE3 A:TRP126 5.0 34.1 1.0 CA A:ALA129 5.0 30.0 1.0 CG A:ARG154 5.0 38.0 1.0

M.L.Quillin, W.A.Breyer, I.J.Griswold, B.W.Matthews. Size Versus Polarizability in Protein-Ligand Interactions: Binding of Noble Gases Within Engineered Cavities in Phage T4 Lysozyme. J.Mol.Biol. V. 302 955 2000.
ISSN: ISSN 0022-2836
PubMed: 10993735
DOI: 10.1006/JMBI.2000.4063